1.
Article
in English
| IMSEAR
| ID: sea-16630
ABSTRACT
Four anti-alphafoetoprotein (AFP) monoclonal antibodies (MAb) were raised in the laboratory and characterized using ELISA and immunodot assays. The affinity constants of the MAbs, analysed by scatchard plots, ranged from 3.1 X 10(8) to 2.15 X 10(9) M/l. Epitope analysis using competition RIA indicated that MAb 5E2D7 and 5E2E3 recognize different epitopes on AFP. This combination was used to set up a two site sandwich ELISA with HRPO conjugated 5E2D7. AFP values in sera of hepatocellular carcinoma patients and pregnant women were quantitated using sandwich ELISA. The anti-AFP MAbs showed strong reactivity when tested on hepatoma tissue sections using immunoperoxidase technique.
Subject(s)
Antibodies, Monoclonal/analysis , Carcinoma, Hepatocellular/analysis , Enzyme-Linked Immunosorbent Assay , Female , Humans , Immunoblotting , Liver Neoplasms/analysis , Pregnancy , alpha-Fetoproteins/immunology
2.
Indian J Exp Biol
;
1984 Dec; 22(12): 657-61
Article
in English
| IMSEAR
| ID: sea-62999
3.
Indian J Exp Biol
;
1984 Dec; 22(12): 662-5
Article
in English
| IMSEAR
| ID: sea-57037
4.
Indian J Biochem Biophys
;
1982 Dec; 19(6): 426-8
Article
in English
| IMSEAR
| ID: sea-28662
5.
Indian J Exp Biol
;
1982 Feb; 20(2): 121-5
Article
in English
| IMSEAR
| ID: sea-59779